Unfortunately these studies did not show a reduction in fat cell number by apoptosis but they did show a marked reduction in fat cell volume, generally speaking it is fat cell number reductions that we want, but still these studies are interesting nonetheless.
Leucine deprivation decreases fat mass by stimulation of lipolysis in white adipose tissue and upregulation of uncoupling protein 1 (UCP1) in brown adipose tissue.
As we recently reported, mice maintained on a leucine-deficient diet for 7 days experienced a dramatic reduction in abdominal fat mass (9).
In our current study, we observed increases in lipolysis and expression of β-oxidation genes and decreases in expression of lipogenic genes and activity of fatty acid synthase (FAS) in WAT,
In addition, we observed for the first time that leucine deprivation increases expression of uncoupling protein (UCP)-1 in brown adipose tissue (BAT), suggesting increased thermogenesis.
The rapid fat loss induced by leucine deprivation suggested a possible increase in energy expenditure.
Serum free fatty acid (FFA) and glycerol levels were much lower in leucine-deprived mice than in pair-fed and control diet–fed groups
Consistent with these findings, no apoptosis was detected by Tdt-mediated dUTP nick end labeling (TUNEL) staining in mice maintained on a leucine-deficient diet
the results of the present study show that leucine deprivation significantly reduces body weight and abdominal adipose mass without affecting proportion of lean body mass
Mice maintained on a leucine-deficient diet, however, reduced their food intake by 15%. These results are consistent with previous studies showing that mice consume less food when provided with a diet deficient in essential amino acids
Fat mobilization in response to increased energy requirements is normally mediated via increased activities of the sympathetic nerve system
Leucine Deprivation Stimulates Fat Loss via Increasing CRH Expression in The Hypothalamus and Activating The Sympathetic Nervous System.
leucine deprivation stimulates fat loss by increasing expression of corticotrophin-releasing hormone in the hypothalamus
the effect of leucine deprivation on fat loss is mediated by activation of the sympathetic nervous system.